Hotspot

accD

LSC · 53935-55401 · 1467 bp

Back to Arenaria

Pi 0.0688

MarkerSeek score 83.50

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1467
variable_sites: 151
pi: 0.0688
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AAAAACTCGGCCCAATCCTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	2425 bp	0.666667	66.6
accD_p2	AAAACTCGGCCCAATCCTTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	2424 bp	0.666667	66.6
accD_p3	AAAAACTCGGCCCAATCCTT	CGCATAAGGGCTCTTTCAGT	45.0	50.0	58.0	58.0	2586 bp	0.666667	66.6
accD_p4	AAAACTCGGCCCAATCCTTT	CGCATAAGGGCTCTTTCAGT	45.0	50.0	58.0	58.0	2585 bp	0.666667	66.6
accD_p5	AAAACTCGGCCCAATCCTTT	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.0	57.9	2430 bp	0.666667	66.0