Hotspot

ndhG-ndhI

SSC · 113028-113405 · 378 bp

Back to Arenaria

Pi 0.171

MarkerSeek score 79.40

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 378
variable_sites: 96
pi: 0.171
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCAATCCCAACGAAAAGGCA	AATTGATCATCTCGGAGCGG	45.0	50.0	58.0	57.8	879-881 bp	0.666667	65.6
ndhG-ndhI_p2	ATAAGGAGCTGTGCTGAAGC	AATTGATCATCTCGGAGCGG	50.0	50.0	58.0	57.8	949-951 bp	0.666667	65.3
ndhG-ndhI_p3	CCAACAGTCCACAGACACAA	AATTGATCATCTCGGAGCGG	50.0	50.0	58.0	57.8	1042-1044 bp	0.666667	65.3
ndhG-ndhI_p4	TCCCAACGAAAAGGCAGAAA	AATTGATCATCTCGGAGCGG	45.0	50.0	57.9	57.8	875-877 bp	0.666667	65.2
ndhG-ndhI_p5	TCAATCCCAACGAAAAGGCA	TCAAATTGCTCTGGGTCGTT	45.0	45.0	58.0	57.7	602-604 bp	0.666667	64.8