Hotspot

rpl20-rps12

LSC · 64492-65254 · 763 bp

Back to Arenaria

Pi 0.0764

MarkerSeek score 84.50

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 763
variable_sites: 87
pi: 0.0764
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	GAACGAACCGAGTCGACTAC	TGTTGATCTTGTAGCGGTCG	55.0	50.0	58.1	58.0	1749-1751 bp	0.666667	66.1
rpl20-rps12_p2	GAACGAACCGAGTCGACTAC	TCGTCAATAGAAGCCCAAGC	55.0	50.0	58.1	58.0	1778-1780 bp	0.666667	66.0
rpl20-rps12_p3	GAACGAACCGAGTCGACTAC	AAGCAACGGAGCCATCATAG	55.0	50.0	58.1	58.0	2231-2233 bp	0.666667	65.8
rpl20-rps12_p4	GAACGAACCGAGTCGACTAC	AATCTCACGAAATCCCCAGC	55.0	50.0	58.1	58.0	1547-1549 bp	0.666667	65.8
rpl20-rps12_p5	GAACGAACCGAGTCGACTAC	TCCCGCGAGATAGAAGAACT	55.0	50.0	58.1	57.9	1991-1993 bp	0.666667	65.7