Hotspot

trnP-UGG-psaJ

LSC · 62112-62503 · 392 bp

Back to Arenaria

Pi 0.119

MarkerSeek score 69.60

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 392
variable_sites: 70
pi: 0.119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	CGGTGCTACGGAAAGATATGT	50.0	47.6	58.1	57.9	438-488 bp	1.0	82.5
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	CGGTGCTACGGAAAGATATGT	50.0	47.6	58.1	57.9	437-487 bp	1.0	82.4
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	CGGTGCTACGGAAAGATATGT	50.0	47.6	58.1	57.9	449-499 bp	1.0	82.4
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	CGGTGCTACGGAAAGATATGT	50.0	47.6	58.1	57.9	448-498 bp	1.0	82.4
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	CGGTGCTACGGAAAGATATGT	50.0	47.6	58.1	57.9	451-501 bp	1.0	82.4