Hotspot

petA-psbJ

LSC · 58734-59581 · 848 bp

Back to Arenaria

Pi 0.122

MarkerSeek score 82.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 848
variable_sites: 152
pi: 0.122
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CTTTTCAGAGCTACCGTCCC	TGGGGTCAATATCCGCAATG	55.0	50.0	58.0	58.0	1680-1870 bp	1.0	91.7
petA-psbJ_p2	CTTTTCAGAGCTACCGTCCC	GGACTAGCTGTACCTACCGT	55.0	55.0	58.0	58.0	1708-1898 bp	1.0	91.5
petA-psbJ_p3	CTTTTCAGAGCTACCGTCCC	GTCTCTAGCACGACCACTTG	55.0	55.0	58.0	58.0	1555-1574 bp	0.666667	67.6
petA-psbJ_p4	CTTTTCAGAGCTACCGTCCC	ACACAATCAAACCCGAACGA	55.0	45.0	58.0	58.0	1776-1795 bp	0.666667	67.2
petA-psbJ_p5	CTTTTCAGAGCTACCGTCCC	GAGCCGACAAGGAATTCCAT	55.0	50.0	58.0	58.0	2020-2039 bp	0.666667	67.1