Hotspot

ndhC-trnV-UAC

LSC · 47438-47988 · 551 bp

Back to Arenaria

Pi 0.271

MarkerSeek score 86.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 551
variable_sites: 220
pi: 0.271
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGCCCAGAAAATGTCATATTCA	AGAAGGTCTACGGTTCGAGT	36.4	50.0	56.0	57.8	612-736 bp	1.0	58.7
ndhC-trnV-UAC_p2	AAAATGCCCAGAAAATGTCA	AGAAGGTCTACGGTTCGAGT	35.0	50.0	53.9	57.8	616-740 bp	1.0	57.7
ndhC-trnV-UAC_p3	AAAAATGCCCAGAAAATGTCA	AGAAGGTCTACGGTTCGAGT	33.3	50.0	54.8	57.8	617-741 bp	1.0	56.7
ndhC-trnV-UAC_p4	TGCCCAGAAAATGTCATATTCA	GAAGGTCTACGGTTCGAGTC	36.4	55.0	56.0	57.5	611-735 bp	1.0	55.9
ndhC-trnV-UAC_p5	AAATGCCCAGAAAATGTCAT	AGAAGGTCTACGGTTCGAGT	35.0	50.0	53.7	57.8	615-739 bp	1.0	55.8