Hotspot

trnE-UUC-trnT-GGU

LSC · 28722-29161 · 440 bp

Back to Arenaria

Pi 0.112

MarkerSeek score 83.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 440
variable_sites: 73
pi: 0.112
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	488-527 bp	1.0	90.8
trnE-UUC-trnT-GGU_p2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	486-525 bp	1.0	90.8
trnE-UUC-trnT-GGU_p3	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	467-506 bp	1.0	90.3
trnE-UUC-trnT-GGU_p4	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	465-504 bp	1.0	90.3
trnE-UUC-trnT-GGU_p5	GATGTCCTGAACCGCTAGAC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	485-524 bp	1.0	90.3