Hotspot

matK-trnK-UUU

LSC · 3152-3854 · 703 bp

Back to Arenaria

Pi 0.0831

MarkerSeek score 83.70

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 703
variable_sites: 86
pi: 0.0831
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CCGAAGTGTTTGAACCAGGA	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.0	58.2	1210-1216 bp	0.666667	65.6
matK-trnK-UUU_p2	CCGAAGTGTTTGAACCAGGA	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.0	58.2	1209-1215 bp	0.666667	65.6
matK-trnK-UUU_p3	AAGACGGGGCATCTTTCATC	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.0	58.2	1235-1241 bp	0.666667	65.5
matK-trnK-UUU_p4	AAGACGGGGCATCTTTCATC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.0	58.2	1234-1240 bp	0.666667	65.5
matK-trnK-UUU_p5	CCGAAGTGTTTGAACCAGGA	ATGGGGGTTGCTAACTCAAC	50.0	50.0	58.0	57.8	1227-1233 bp	0.666667	65.2