Hotspot

rps19

LSC · 86488-86766 · 279 bp

Back to Areca

Pi 0.00179
MarkerSeek score 27.00
Primer available yes
Label rps19

Feature Metadata

label_name
rps19
feature_type
gene
region
LSC
length_bp
279
variable_sites
1
pi
0.00179
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
27.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19_p1 GCGAACCAAAAGGAACGATG GGGTAGAGCCGGATCTAAGT 50.0 55.0 58.0 58.0 1407-1413 bp 1.0 80.7
rps19_p2 GCGAACCAAAAGGAACGATG TGGGTAGAGCCGGATCTAAG 50.0 55.0 58.0 58.0 1408-1414 bp 1.0 80.6
rps19_p3 GCGAACCAAAAGGAACGATG GTTATCCTGCGCTTGGAAGA 50.0 50.0 58.0 58.0 1270-1276 bp 1.0 80.6
rps19_p4 TGCTGCTGCAGAATAAACCA GGGTAGAGCCGGATCTAAGT 45.0 55.0 58.1 58.0 1092-1098 bp 1.0 80.5
rps19_p5 GCGAACCAAAAGGAACGATG GAGGGTAAATCGGCCACATT 50.0 50.0 58.0 57.9 1520-1526 bp 1.0 80.2