Hotspot

psbB

LSC · 74649-76175 · 1527 bp

Back to Areca

Pi 0.00273
MarkerSeek score 60.50
Primer available yes
Label psbB

Feature Metadata

label_name
psbB
feature_type
gene
region
LSC
length_bp
1527
variable_sites
8
pi
0.00273
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB_p1 AGTATAGTCCTTTCCAATGCGA GGCGAAAGATACCAGAGCAA 40.9 50.0 57.0 58.0 1626 bp 1.0 56.0
psbB_p2 AGTATAGTCCTTTCCAATGCGA GAGGCGAAAGATACCAGAGC 40.9 55.0 57.0 58.1 1628 bp 1.0 55.1
psbB_p3 AGTCCTTTCCAATGCGATAA GGCGAAAGATACCAGAGCAA 40.0 50.0 54.7 58.0 1621 bp 1.0 54.1
psbB_p4 AGTCCTTTCCAATGCGATAA GAGGCGAAAGATACCAGAGC 40.0 55.0 54.7 58.1 1623 bp 1.0 53.2
psbB_p5 AGTCCTTTCCAATGCGATAAA GGCGAAAGATACCAGAGCAA 38.1 50.0 55.6 58.0 1621 bp 1.0 52.8