Hotspot

psbA

LSC · 151-1212 · 1062 bp

Pi 0.000942

MarkerSeek score 39.20

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 2
pi: 0.000942
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TGCTCCATTGTTTGATTCAACG	GTGCTTGGGAGTCCTTGAAA	40.9	50.0	58.1	58.0	1152 bp	1.0	63.2
psbA_p2	GCTCCATTGTTTGATTCAACGA	GTGCTTGGGAGTCCTTGAAA	40.9	50.0	57.8	58.0	1151 bp	1.0	62.7
psbA_p3	TGCTCCATTGTTTGATTCAACG	AATACGTGTGCTTGGGAGTC	40.9	50.0	58.1	57.9	1159 bp	1.0	62.6
psbA_p4	TGGGTATTGCTCCATTGTTTGA	GTGCTTGGGAGTCCTTGAAA	40.9	50.0	58.2	58.0	1159 bp	1.0	62.2
psbA_p5	GCTCCATTGTTTGATTCAACGA	AATACGTGTGCTTGGGAGTC	40.9	50.0	57.8	57.9	1158 bp	1.0	62.1