Hotspot

psaC-ndhE

SSC · 120198-120797 · 600 bp

Back to Areca

Pi 0.00926
MarkerSeek score 69.90
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
600
variable_sites
10
pi
0.00926
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 ACATCCGTCCCAAGGTATCA CTGAAGCAGCTATTGGGCTA 50.0 50.0 58.1 57.4 725-812 bp 1.0 79.0
psaC-ndhE_p2 ACATCCGTCCCAAGGTATCA TGAAGCAGCTATTGGGCTAG 50.0 50.0 58.1 57.4 724-811 bp 1.0 79.0
psaC-ndhE_p3 ACATCCGTCCCAAGGTATCA TGTTATAGCTGTTGCGGCTG 50.0 50.0 58.1 58.6 745-832 bp 1.0 78.9
psaC-ndhE_p4 ACATCCGTCCCAAGGTATCA GAAGCAGCTATTGGGCTAGC 50.0 55.0 58.1 58.8 723-810 bp 1.0 77.9
psaC-ndhE_p5 CAATACATCTGTGGGGCAGG TGAAGCAGCTATTGGGCTAG 55.0 50.0 58.3 57.4 700-787 bp 1.0 77.5