Hotspot

petD-rpoA

LSC · 80020-80211 · 192 bp

Back to Areca

Pi 0.00611
MarkerSeek score 61.40
Primer available yes
Label petD-rpoA

Feature Metadata

label_name
petD-rpoA
feature_type
igs
region
LSC
length_bp
192
variable_sites
2
pi
0.00611
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD-rpoA_p1 TCGGTACCGTAGTAGCTCTT ACACTCTACAGAAGTATTTCGCA 50.0 39.1 57.3 57.6 352-375 bp 1.0 51.7
petD-rpoA_p2 ATCGGTACCGTAGTAGCTCT ACACTCTACAGAAGTATTTCGCA 50.0 39.1 57.1 57.6 353-376 bp 1.0 49.9
petD-rpoA_p3 TCGGTACCGTAGTAGCTCTTT ACACTCTACAGAAGTATTTCGCA 47.6 39.1 58.0 57.6 352-375 bp 1.0 49.3
petD-rpoA_p4 CGGTACCGTAGTAGCTCTTTG ACACTCTACAGAAGTATTTCGCA 52.4 39.1 57.9 57.6 351-374 bp 1.0 48.8
petD-rpoA_p5 ATCGGTACCGTAGTAGCTCTT ACACTCTACAGAAGTATTTCGCA 47.6 39.1 57.8 57.6 353-376 bp 1.0 47.7