Hotspot

petA-psbJ

LSC · 64719-65742 · 1024 bp

Back to Areca

Pi 0.0192
MarkerSeek score 68.10
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1024
variable_sites
35
pi
0.0192
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC GGTTCATATTCCGGGTTGGG 50.0 55.0 58.0 58.3 1176-1184 bp 1.0 79.6
petA-psbJ_p2 GGCCTTTTGTTCTTCTTGGC GGTTCATATTCCGGGTTGGG 50.0 55.0 57.8 58.3 1159-1167 bp 1.0 78.7
petA-psbJ_p3 ATCCATTACGTGTCCAAGGC CATATTCCGGGTTGGGTTCA 50.0 50.0 58.0 57.6 1172-1180 bp 1.0 78.6
petA-psbJ_p4 ATCCATTACGTGTCCAAGGC GTTCATATTCCGGGTTGGGT 50.0 50.0 58.0 57.6 1175-1183 bp 1.0 78.6
petA-psbJ_p5 CATTACGTGTCCAAGGCCTT GGTTCATATTCCGGGTTGGG 50.0 55.0 58.2 58.3 1173-1181 bp 1.0 78.5