Hotspot

accD-psaI

LSC · 60638-61609 · 972 bp

Back to Areca

Pi 0.013
MarkerSeek score 63.50
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
972
variable_sites
24
pi
0.013
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 CCACGGTTTCTTTCCCTTGA CCATTGCAATTGCCGGAAAT 50.0 45.0 58.0 58.0 1085-1130 bp 1.0 81.5
accD-psaI_p2 TCCACGGTTTCTTTCCCTTG CCATTGCAATTGCCGGAAAT 50.0 45.0 58.0 58.0 1086-1131 bp 1.0 81.5
accD-psaI_p3 CCACGGTTTCTTTCCCTTGA AAGAAGCCATTGCAATTGCC 50.0 45.0 58.0 57.9 1091-1136 bp 1.0 80.8
accD-psaI_p4 TCCACGGTTTCTTTCCCTTG AAGAAGCCATTGCAATTGCC 50.0 45.0 58.0 57.9 1092-1137 bp 1.0 80.8
accD-psaI_p5 TCAGCTCCACGGTTTCTTTC CCATTGCAATTGCCGGAAAT 50.0 45.0 58.1 58.0 1091-1136 bp 1.0 80.7