Hotspot

psbI-trnS-GCU

LSC · 8213-8350 · 138 bp

Back to Areca

Pi 0.0121
MarkerSeek score 60.70
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
138
variable_sites
3
pi
0.0121
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 ATGATCCAGGGCGTAATCCT TAAAGCGGCGGATTGCTAAT 50.0 45.0 58.3 58.0 241 bp 1.0 78.6
psbI-trnS-GCU_p2 ATGATCCAGGGCGTAATCCT GGGTTCGAATCCCTCTCTCT 50.0 55.0 58.3 58.2 196 bp 1.0 77.4
psbI-trnS-GCU_p3 ATGATCCAGGGCGTAATCCT GGATGGGAATGGGGAGAGAT 50.0 55.0 58.3 58.2 273 bp 1.0 76.8
psbI-trnS-GCU_p4 ATGATCCAGGGCGTAATCCT TTCGAATCCCTCTCTCTCCG 50.0 55.0 58.3 58.3 193 bp 1.0 76.6
psbI-trnS-GCU_p5 AATGATCCAGGGCGTAATCC TAAAGCGGCGGATTGCTAAT 50.0 45.0 57.4 58.0 242 bp 1.0 76.1