Hotspot

rpl2

IRa · 153335-154814 · 1480 bp

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Pi 0.00422

MarkerSeek score 43.40

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRa
length_bp: 1480
variable_sites: 20
pi: 0.00422
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	GAATGGTTAAAGCGCCCAAC	GCCCAATTTGTGACCTACCA	50.0	50.0	58.0	58.1	2272-2286 bp	0.375	55.1
rpl2_p2	CGGGAAAGGGGAGAAGAATG	GCCCAATTTGTGACCTACCA	55.0	50.0	58.0	58.1	1885-1886 bp	0.375	54.9
rpl2_p3	AGCATTGGGTCGAACTCTTC	GCCCAATTTGTGACCTACCA	50.0	50.0	57.9	58.1	1945-1946 bp	0.375	54.4
rpl2_p4	GCATTGGGTCGAACTCTTCT	GCCCAATTTGTGACCTACCA	50.0	50.0	57.9	58.1	1944-1945 bp	0.375	54.4
rpl2_p5	GAATGGTTAAAGCGCCCAAC	TCGCCCAATTTGTGACCTAC	50.0	50.0	58.0	58.2	2274-2288 bp	0.375	54.3