Hotspot

ndhD

SSC · 116183-117706 · 1524 bp

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Pi 0.00534

MarkerSeek score 48.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1524
variable_sites: 24
pi: 0.00534
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTGGACCGTATTCGCGATTT	TCAGCTCCAAGAACAGAGGA	45.0	50.0	58.0	58.0	2200-2235 bp	1.0	82.3
ndhD_p2	TTACTTGGACCGTATTCGCG	TCAGCTCCAAGAACAGAGGA	50.0	50.0	58.1	58.0	2204-2239 bp	1.0	81.7
ndhD_p3	TTGGACCGTATTCGCGATTT	AACAACTCGTAGCATGGGTC	45.0	50.0	58.0	57.9	2105-2140 bp	1.0	81.6
ndhD_p4	TTGGACCGTATTCGCGATTT	CTTGGGACGGTTGTAAAGCT	45.0	50.0	58.0	58.1	2232-2267 bp	1.0	81.6
ndhD_p5	TTGGACCGTATTCGCGATTT	TGTGAATCTGCCTGTCCAAC	45.0	50.0	58.0	58.1	2161-2196 bp	1.0	81.5