Hotspot

ndhB-trnL-CAA

IRa · 144748-145317 · 570 bp

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Pi 0.0205

MarkerSeek score 71.10

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: IRa
length_bp: 570
variable_sites: 43
pi: 0.0205
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	554-630 bp	1.0	89.1
ndhB-trnL-CAA_p2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	552-628 bp	1.0	89.1
ndhB-trnL-CAA_p3	TGCAATTGCTCAGGATACCC	TACCATTTCACCACCAAGGC	50.0	50.0	57.9	58.1	542-618 bp	1.0	88.6
ndhB-trnL-CAA_p4	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	50.0	50.0	57.9	58.2	549-625 bp	1.0	87.6
ndhB-trnL-CAA_p5	TGCAATTGCTCAGGATACCC	TCTCGCGTGTCTACCATTTC	50.0	50.0	57.9	57.7	553-629 bp	1.0	86.9