Hotspot

rpl32-trnL-UAG

SSC · 114014-114775 · 762 bp

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Pi 0.0297
MarkerSeek score 69.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
762
variable_sites
53
pi
0.0297
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGTGCGACAAACAAATAAGT TCCTAAGAGCAGCGTGTCTA 35.0 50.0 54.1 57.9 817-860 bp 1.0 53.4
rpl32-trnL-UAG_p2 TGTGCGACAAACAAATAAGT AGCAGCGTGTCTACCAATTT 35.0 45.0 54.1 57.8 810-853 bp 1.0 53.0
rpl32-trnL-UAG_p3 TGTGCGACAAACAAATAAGT CCTAAGAGCAGCGTGTCTAC 35.0 55.0 54.1 57.8 816-859 bp 1.0 52.8
rpl32-trnL-UAG_p4 TGTGCGACAAACAAATAAGT CTAAGAGCAGCGTGTCTACC 35.0 55.0 54.1 57.8 815-858 bp 1.0 52.8
rpl32-trnL-UAG_p5 TGTGCGACAAACAAATAAGT GAGCAGCGTGTCTACCAATT 35.0 50.0 54.1 58.3 811-854 bp 1.0 52.2