Hotspot

ndhF-rpl32

SSC · 112934-113851 · 918 bp

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Pi 0.0135

MarkerSeek score 62.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 918
variable_sites: 33
pi: 0.0135
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAACAAAAGGT	GCTTTTTCCAAATATTTTTACGA	45.0	26.1	55.8	52.6	752-1082 bp	1.0	46.7
ndhF-rpl32_p2	ACAAAAGGTATTATCCACGCG	GCTTTTTCCAAATATTTTTACGA	42.9	26.1	56.7	52.6	741-1071 bp	1.0	46.7
ndhF-rpl32_p3	CAAAAGGTATTATCCACGCGT	GCTTTTTCCAAATATTTTTACGA	42.9	26.1	56.7	52.6	740-1070 bp	1.0	46.7
ndhF-rpl32_p4	ACAAAAGGTATTATCCACGCGT	GCTTTTTCCAAATATTTTTACGA	40.9	26.1	58.4	52.6	741-1071 bp	1.0	46.7
ndhF-rpl32_p5	CTGGAAGTGGAACAAAAGGT	GCTTTTTCCAAATATTTTTACGAA	45.0	25.0	55.8	53.4	752-1082 bp	1.0	46.7