Hotspot

petL-petG

LSC · 66364-66535 · 172 bp

Back to Ardisia

Pi 0.023

MarkerSeek score 61.00

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 172
variable_sites: 12
pi: 0.023
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TAGCTCCTTCATGCCTACGA	CAACTGATCACCACGTCTGT	50.0	50.0	57.9	57.8	375-383 bp	1.0	82.4
petL-petG_p2	ATTGGTCTGAGCAAGGTACG	CAACTGATCACCACGTCTGT	50.0	50.0	57.6	57.8	299-307 bp	1.0	80.9
petL-petG_p3	TAGCTCCTTCATGCCTACGA	CCAACTGATCACCACGTCTG	50.0	55.0	57.9	58.6	376-384 bp	1.0	79.1
petL-petG_p4	TAGCTCCTTCATGCCTACGA	GTCCAACTGATCACCACGTC	50.0	55.0	57.9	58.6	378-386 bp	1.0	79.1
petL-petG_p5	TAGCTCCTTCATGCCTACGA	AAGGTCCAACTGATCACCAC	50.0	50.0	57.9	57.4	381-389 bp	1.0	79.0