Hotspot

ndhC-trnV-UAC

LSC · 50949-51439 · 491 bp

Back to Ardisia

Pi 0.0237
MarkerSeek score 63.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
491
variable_sites
32
pi
0.0237
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ATCATATTCATAAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 26.1 55.0 52.2 58.7 536-1186 bp 1.0 45.1
ndhC-trnV-UAC_p2 TCATATTCATAAAGCAGAAACAT GTTCGAGTCCGTATAGCCCT 26.1 55.0 52.2 58.7 535-1185 bp 1.0 45.1
ndhC-trnV-UAC_p3 ATCATATTCATAAAGCAGAAACA TTCGAGTCCGTATAGCCCTA 26.1 50.0 52.2 57.1 535-1185 bp 1.0 45.1
ndhC-trnV-UAC_p4 TCATATTCATAAAGCAGAAACAT TTCGAGTCCGTATAGCCCTA 26.1 50.0 52.2 57.1 534-1184 bp 1.0 45.1
ndhC-trnV-UAC_p5 ATCATATTCATAAAGCAGAAACAT GTTCGAGTCCGTATAGCCCT 25.0 55.0 52.9 58.7 536-1186 bp 1.0 45.1