Hotspot

trnT-GGU-psbD

LSC · 31782-33067 · 1286 bp

Back to Ardisia

Pi 0.0299

MarkerSeek score 83.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1286
variable_sites: 130
pi: 0.0299
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1313-1545 bp	1.0	89.6
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1316-1548 bp	1.0	89.4
trnT-GGU-psbD_p3	CGACATAGCGGCTCCATTAA	GGACCAGCCTACAAAAACGA	50.0	50.0	57.9	58.1	1364-1596 bp	1.0	88.7
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1303-1535 bp	1.0	88.5
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1306-1538 bp	1.0	88.4