Hotspot

rps16-trnQ-UUG

LSC · 5982-7467 · 1486 bp

Back to Ardisia

Pi 0.0106

MarkerSeek score 61.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1486
variable_sites: 46
pi: 0.0106
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	ATCATGTCTTTCAAGCCGCA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.2	58.1	1571-1916 bp	1.0	81.6
rps16-trnQ-UUG_p2	GATCATGTCTTTCAAGCCGC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	1572-1917 bp	1.0	79.3
rps16-trnQ-UUG_p3	TGGATGTAAGTCCACAACGG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	1591-1936 bp	1.0	79.2
rps16-trnQ-UUG_p4	ATCATGTCTTTCAAGCCGCA	AAATTTAAATGGGGCGTGGC	45.0	45.0	58.2	57.6	1628-1973 bp	1.0	78.9
rps16-trnQ-UUG_p5	CCAAGAGCACCTTCATCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	1622-1967 bp	1.0	78.8