Hotspot

ycf1

SSC · 108380-113575 · 5196 bp

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Pi 0.000385

MarkerSeek score 52.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5196
variable_sites: 2
pi: 0.000385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCCCTTTTGAATGGGATGT	TATGACCATCGAGGGGGTTT	45.0	50.0	56.7	58.1	306 bp	1.0	68.8
ycf1_p2	TGAATGGGATGTATCGTGGA	TATGACCATCGAGGGGGTTT	45.0	50.0	56.0	58.1	298 bp	1.0	63.5
ycf1_p3	TGGGATGTATCGTGGAAGAA	TATGACCATCGAGGGGGTTT	45.0	50.0	55.9	58.1	294 bp	1.0	62.9
ycf1_p4	TGAATGGGATGTATCGTGGAAG	TATGACCATCGAGGGGGTTT	45.5	50.0	57.9	58.1	298 bp	1.0	62.8
ycf1_p5	TGGGATGTATCGTGGAAGAATG	TATGACCATCGAGGGGGTTT	45.5	50.0	57.9	58.1	294 bp	1.0	62.8