Hotspot

psbA

LSC · 467-1528 · 1062 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GAGCAATAACGCCCTCTTGA	TTGAAAATTCGTGCGCTTGG	50.0	45.0	58.0	58.2	1203 bp	1.0	77.9
psbA_p2	AAAAGGAGCAATAACGCCCT	TTGAAAATTCGTGCGCTTGG	45.0	45.0	57.8	58.2	1208 bp	1.0	76.4
psbA_p3	GAGCAATAACGCCCTCTTGA	ATATTGGTTGACACGGGCAT	50.0	45.0	58.0	57.6	1268 bp	1.0	76.3
psbA_p4	GAGCAATAACGCCCTCTTGA	ATTGGTTGACACGGGCATAT	50.0	45.0	58.0	57.6	1266 bp	1.0	76.3
psbA_p5	AAAGGAGCAATAACGCCCTC	TTGAAAATTCGTGCGCTTGG	50.0	45.0	58.2	58.2	1207 bp	1.0	76.1