Hotspot

matK

LSC · 2057-3577 · 1521 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1521
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	ACATACTACATCAACATTTCAGA	ACCCCCAAAATCCTCTACCT	30.4	50.0	53.5	57.6	1680-1681 bp	1.0	40.0
matK_p2	ACATACTACATCAACATTTCAGAA	ACCCCCAAAATCCTCTACCT	29.2	50.0	54.3	57.6	1680-1681 bp	1.0	40.0
matK_p3	ACATACTACATCAACATTTCAGAAT	ACCCCCAAAATCCTCTACCT	28.0	50.0	54.8	57.6	1680-1681 bp	1.0	40.0
matK_p4	CTACATACTACATCAACATTTCAGA	ACCCCCAAAATCCTCTACCT	32.0	50.0	54.7	57.6	1682-1683 bp	1.0	40.0
matK_p5	ACATACTACATCAACATTTCAGA	AACCCCCAAAATCCTCTACC	30.4	50.0	53.5	56.8	1681-1682 bp	1.0	40.0