Hotspot

ycf1

IRb · 111562-112710 · 1149 bp

Back to Archiphysalis

Pi 0.00087

MarkerSeek score 59.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1149
variable_sites: 1
pi: 0.00087
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACAGATGTCGTTTCTAGTCT	GCGGACGCATCTCTTCTTAT	40.0	50.0	53.5	57.9	1342 bp	1.0	42.6
ycf1_p2	AACAGATGTCGTTTCTAGTCT	GCGGACGCATCTCTTCTTAT	38.1	50.0	54.4	57.9	1343 bp	1.0	41.8
ycf1_p3	ACATAACAGATGTCGTTTCTAGTCT	GCGGACGCATCTCTTCTTAT	36.0	50.0	57.8	57.9	1347 bp	1.0	40.1
ycf1_p4	AGAAATAAGGGAGGTTTGTG	GCGGACGCATCTCTTCTTAT	40.0	50.0	52.7	57.9	1247 bp	1.0	40.1
ycf1_p5	ACAGATGTCGTTTCTAGTCTATCTC	GCGGACGCATCTCTTCTTAT	40.0	50.0	57.7	57.9	1342 bp	1.0	40.1