Hotspot

rps16

LSC · 5097-6218 · 1122 bp

Back to Archiphysalis

Pi 0.00178

MarkerSeek score 59.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1122
variable_sites: 2
pi: 0.00178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACAAATCACTACCCCCTTTT	CATCAGCCCAACCAATGACT	40.0	50.0	54.4	58.2	1237 bp	1.0	50.1
rps16_p2	ACAAATCACTACCCCCTTTTT	CATCAGCCCAACCAATGACT	38.1	50.0	55.3	58.2	1237 bp	1.0	49.0
rps16_p3	ACAAATCACTACCCCCTTTT	ACATCAGCCCAACCAATGAC	40.0	50.0	54.4	58.5	1238 bp	1.0	47.7
rps16_p4	AGAAAAGGAAAGGGAGGAAA	CATCAGCCCAACCAATGACT	40.0	50.0	54.0	58.2	1296 bp	1.0	46.9
rps16_p5	ACAAATCACTACCCCCTTTTT	ACATCAGCCCAACCAATGAC	38.1	50.0	55.3	58.5	1238 bp	1.0	46.6