Hotspot

ndhF

SSC · 112700-114922 · 2223 bp

Back to Archiphysalis

Pi 0.00135

MarkerSeek score 43.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 3
pi: 0.00135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGGGTGGAAAGTGAGGAAGA	TGAATCCCCGTTCGATTCAC	50.0	50.0	57.9	58.0	2880 bp	1.0	79.0
ndhF_p2	AGGGTGGAAAGTGAGGAAGA	AATCCCCGTTCGATTCACTG	50.0	50.0	57.9	58.0	2878 bp	1.0	79.0
ndhF_p3	ACTGCTTCCGAAATGAAGGG	TGAATCCCCGTTCGATTCAC	50.0	50.0	58.2	58.0	2841 bp	1.0	78.6
ndhF_p4	ACTGCTTCCGAAATGAAGGG	AATCCCCGTTCGATTCACTG	50.0	50.0	58.2	58.0	2839 bp	1.0	78.5
ndhF_p5	TAGGCAGAATACCCTCACCC	TGAATCCCCGTTCGATTCAC	55.0	50.0	58.2	58.0	2942 bp	1.0	78.4