Hotspot

ndhA

SSC · 122352-124610 · 2259 bp

Back to Archiphysalis

Pi 0.00221

MarkerSeek score 58.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2259
variable_sites: 5
pi: 0.00221
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTTTGAGACAAGTCGTGAA	TGGGAGAAGTTGATCGTTGA	40.0	45.0	55.2	56.5	2319-2323 bp	1.0	45.6
ndhA_p2	TTGTTTGAGACAAGTCGTGA	TGGGAGAAGTTGATCGTTGA	40.0	45.0	55.2	56.5	2320-2324 bp	1.0	45.6
ndhA_p3	TTGTTTGAGACAAGTCGTGAA	TGGGAGAAGTTGATCGTTGA	38.1	45.0	56.0	56.5	2320-2324 bp	1.0	44.0
ndhA_p4	TGTTTGAGACAAGTCGTGAATC	TGGGAGAAGTTGATCGTTGA	40.9	45.0	56.9	56.5	2319-2323 bp	1.0	43.6
ndhA_p5	TGTTTGAGACAAGTCGTGAA	TGGGAGAAGTTGATCGTTGAA	40.0	42.9	55.2	57.2	2319-2323 bp	1.0	43.6