Hotspot

ndhC-trnV-UAC

LSC · 52817-53961 · 1145 bp

Back to Archiphysalis

Pi 0.000874
MarkerSeek score 59.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1145
variable_sites
1
pi
0.000874
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TTGCTAATCGGGGCTAACAC AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.0 57.8 1284-1285 bp 1.0 78.3
ndhC-trnV-UAC_p2 GCTAATCGGGGCTAACACTC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.1 57.8 1282-1283 bp 1.0 77.5
ndhC-trnV-UAC_p3 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1287-1288 bp 1.0 75.8
ndhC-trnV-UAC_p4 TTGCTAATCGGGGCTAACAC GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.0 57.5 1283-1284 bp 1.0 75.5
ndhC-trnV-UAC_p5 GGCTAACACTCCGGAAATGA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.6 57.8 1273-1274 bp 1.0 75.4