Hotspot

petN-psbM

LSC · 29600-30713 · 1114 bp

Back to Archiphysalis

Pi 0.00898

MarkerSeek score 63.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1114
variable_sites: 10
pi: 0.00898
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	AGTTCCTACCGCTTTTCTACT	45.0	42.9	58.0	56.4	1248 bp	1.0	59.7
petN-psbM_p2	AGTAAGTCTTGCTTGGGCTG	AGTTCCTACCGCTTTTCTACT	50.0	42.9	57.8	56.4	1254 bp	1.0	58.2
petN-psbM_p3	GGGGAAGGAGTGGACTCTAG	AGTTCCTACCGCTTTTCTACT	60.0	42.9	58.2	56.4	1192 bp	1.0	58.0
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	AGTTCCTACCGCTTTTCTACTT	45.0	40.9	58.0	57.1	1248 bp	1.0	57.5
petN-psbM_p5	TCTTGCTTGGGCTGCTTTAA	TCTAGTTCCTACCGCTTTTCT	45.0	42.9	58.0	56.1	1251 bp	1.0	57.5