Hotspot

rps18

LSC · 75636-75944 · 309 bp

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Pi 0.00971

MarkerSeek score 48.90

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 309
variable_sites: 3
pi: 0.00971
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	TCACGATAAGGAATAAACAAACT	CGAACAAAACATCAATCCACGT	30.4	40.9	54.1	58.1	400 bp	1.0	40.6
rps18_p2	TCACGATAAGGAATAAACAAACT	TCGAACAAAACATCAATCCACG	30.4	40.9	54.1	57.9	401 bp	1.0	40.6
rps18_p3	TTCACGATAAGGAATAAACAAACT	CGAACAAAACATCAATCCACGT	29.2	40.9	54.8	58.1	401 bp	1.0	40.6
rps18_p4	TTCACGATAAGGAATAAACAAACT	TCGAACAAAACATCAATCCACG	29.2	40.9	54.8	57.9	402 bp	1.0	40.6
rps18_p5	TCACGATAAGGAATAAACAAACT	CGAACAAAACATCAATCCACG	30.4	42.9	54.1	56.4	400 bp	1.0	40.6