Hotspot

trnC-GCA-petN

LSC · 31303-32835 · 1533 bp

Back to Archidendron

Pi 0.0188

MarkerSeek score 63.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1533
variable_sites: 28
pi: 0.0188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	1572-1589 bp	1.0	81.6
trnC-GCA-petN_p2	GTATCATTTTGGCGGCATGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.9	58.2	1624-1641 bp	1.0	81.0
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	1633-1650 bp	1.0	79.9
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TCTTCCCCATACTACGAGGG	50.0	55.0	58.1	57.6	1623-1640 bp	1.0	79.7
trnC-GCA-petN_p5	GTATCATTTTGGCGGCATGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	57.9	57.6	1685-1702 bp	1.0	79.3