Hotspot

trnS-GCU-trnG-UCC

LSC · 9230-10682 · 1453 bp

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Pi 0.0176
MarkerSeek score 71.20
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1453
variable_sites
25
pi
0.0176
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TGAATCAAACGAGGGATCCC 50.0 50.0 58.0 57.3 1565-1599 bp 1.0 77.4
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TGAATCAAACGAGGGATCCC 50.0 50.0 58.0 57.3 1564-1598 bp 1.0 77.4
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA TGAATCAAACGAGGGATCCC 50.0 50.0 58.2 57.3 1619-1653 bp 1.0 76.1
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA GGATCCATAGAACGAATCGCA 50.0 47.6 58.0 58.0 1533-1567 bp 1.0 74.9
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC GGATCCATAGAACGAATCGCA 50.0 47.6 58.0 58.0 1532-1566 bp 1.0 74.9