Hotspot

psbI-trnS-GCU

LSC · 8731-9141 · 411 bp

Back to Archidendron

Pi 0.0321

MarkerSeek score 74.60

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 411
variable_sites: 13
pi: 0.0321
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CCTATCTAACGATCCAGGACG	TGGACTAAAGCGTCGGATTG	52.4	50.0	57.2	58.0	522-527 bp	1.0	68.2
psbI-trnS-GCU_p2	CCTATCTAACGATCCAGGACG	GTGGACTAAAGCGTCGGATT	52.4	50.0	57.2	58.0	523-528 bp	1.0	68.2
psbI-trnS-GCU_p3	CCTATCTAACGATCCAGGACG	GCCCTTTTCAATTGGGGAGA	52.4	50.0	57.2	58.1	552-557 bp	1.0	67.6
psbI-trnS-GCU_p4	CCTATCTAACGATCCAGGACG	TTTTTCGTACCGAGGGTTCG	52.4	50.0	57.2	58.2	485-490 bp	1.0	66.7
psbI-trnS-GCU_p5	CCTATCTAACGATCCAGGACG	CAATTGGGGAGAGATGGCTG	52.4	55.0	57.2	58.3	544-549 bp	1.0	65.6