Hotspot

rps16-trnQ-UUG

LSC · 6774-7475 · 702 bp

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Pi 0.0134

MarkerSeek score 70.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 702
variable_sites: 9
pi: 0.0134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	756-782 bp	1.0	83.3
rps16-trnQ-UUG_p2	ATGTCTTTCAAGTCGCACGT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	758-784 bp	1.0	82.3
rps16-trnQ-UUG_p3	GCACGTTGCTTTTTACCACA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	744-770 bp	1.0	81.8
rps16-trnQ-UUG_p4	CATGTCTTTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	759-785 bp	1.0	81.6
rps16-trnQ-UUG_p5	GTCTTTCAAGTCGCACGTTG	AAATAAAAATGGGGCGTGGC	50.0	45.0	58.0	57.6	813-839 bp	1.0	80.5