Hotspot

trnD-GUC

LSC · 30703-30776 · 74 bp

Back to Archidasyphyllum

Pi 0.0135

MarkerSeek score 60.30

Primer available yes

Label trnD-GUC

Feature Metadata

label_name: trnD-GUC
feature_type: tRNA
region: LSC
length_bp: 74
variable_sites: 1
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC_p1	CACAGAAAATGGAGAGGCGA	TGGCCAATCTACCATCAGATG	50.0	47.6	57.9	57.5	219 bp	1.0	67.6
trnD-GUC_p2	TCACAGAAAATGGAGAGGCG	TGGCCAATCTACCATCAGATG	50.0	47.6	57.9	57.5	220 bp	1.0	67.6
trnD-GUC_p3	CACAGAAAATGGAGAGGCGA	CCAACAATTGGCCAATCTACC	50.0	47.6	57.9	57.5	227 bp	1.0	67.4
trnD-GUC_p4	TCACAGAAAATGGAGAGGCG	CCAACAATTGGCCAATCTACC	50.0	47.6	57.9	57.5	228 bp	1.0	67.4
trnD-GUC_p5	TCCCTGTCCCTTCTTTCACA	TGGCCAATCTACCATCAGATG	50.0	47.6	58.2	57.5	235 bp	1.0	66.9