Hotspot

rpoA

LSC · 77354-78178 · 825 bp

Back to Archidasyphyllum

Pi 0

MarkerSeek score 62.40

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 825
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	CTGGAAGGCAATTCTGATTGG	CATAATGGCTGTAGACCCCC	47.6	55.0	57.2	57.4	934-965 bp	1.0	63.7
rpoA_p2	TGGAAGGCAATTCTGATTGG	CATAATGGCTGTAGACCCCC	45.0	55.0	56.0	57.4	933-964 bp	1.0	61.9
rpoA_p3	CTGGAAGGCAATTCTGATTGG	ATAATGGCTGTAGACCCCCG	47.6	55.0	57.2	58.9	933-964 bp	1.0	60.7
rpoA_p4	TTGGACCTTTTGAGGCAATT	CATAATGGCTGTAGACCCCC	40.0	55.0	55.7	57.4	961-992 bp	1.0	59.7
rpoA_p5	TTTGGACCTTTTGAGGCAAT	CATAATGGCTGTAGACCCCC	40.0	55.0	55.7	57.4	962-993 bp	1.0	59.7