Hotspot

psbB

LSC · 71715-73241 · 1527 bp

Back to Archidasyphyllum

Pi 0

MarkerSeek score 34.50

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	GGGGGTCCATAACATAGTCA	TCCCGGATAAAGAAAGAGCA	50.0	45.0	56.0	56.2	1731 bp	0.5	29.8
psbB_p2	CCCTTGCGTTGAAATAATCCA	TCCCGGATAAAGAAAGAGCA	42.9	45.0	56.9	56.2	1760 bp	0.5	28.8
psbB_p3	ACCCTTGCGTTGAAATAATCC	TCCCGGATAAAGAAAGAGCA	42.9	45.0	56.9	56.2	1761 bp	0.5	28.8
psbB_p4	CCCTTGCGTTGAAATAATCCAC	TCCCGGATAAAGAAAGAGCA	45.5	45.0	58.2	56.2	1760 bp	0.5	28.1
psbB_p5	GGGGGTCCATAACATAGTCA	TCCCGGATAAAGAAAGAGCAA	50.0	42.9	56.0	57.0	1731 bp	0.5	28.0