Hotspot

ndhD

SSC · 114259-115761 · 1503 bp

Back to Archidasyphyllum

Pi 0

MarkerSeek score 28.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TCATTAGCCAACCAACTCTT	TTTTTGGGCACGGGTTTTTC	40.0	45.0	54.5	58.0	1592 bp	1.0	52.2
ndhD_p2	TTCATTAGCCAACCAACTCT	TTTTTGGGCACGGGTTTTTC	40.0	45.0	54.5	58.0	1593 bp	1.0	52.2
ndhD_p3	TTCATTAGCCAACCAACTCTT	TTTTTGGGCACGGGTTTTTC	38.1	45.0	55.4	58.0	1593 bp	1.0	51.0
ndhD_p4	TTTCATTAGCCAACCAACTCT	TTTTTGGGCACGGGTTTTTC	38.1	45.0	55.4	58.0	1594 bp	1.0	51.0
ndhD_p5	TCATTAGCCAACCAACTCTT	CGGGTTTTTCTGGTCCAAGT	40.0	50.0	54.5	58.3	1582 bp	1.0	49.9