Hotspot

rpl20-rps12

LSC · 68218-68961 · 744 bp

Back to Archidasyphyllum

Pi 0

MarkerSeek score 59.70

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 744
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCTCCGAGCGATATATCCCC	CAGCGTCGAGGAACATGTAC	55.0	55.0	57.9	58.7	781-810 bp	1.0	76.6
rpl20-rps12_p2	TCTCCGAGCGATATATCCCC	AGCGTCGAGGAACATGTACT	55.0	50.0	57.9	58.8	780-809 bp	1.0	75.9
rpl20-rps12_p3	TCTCCGAGCGATATATCCCC	TCAGCGTCGAGGAACATGTA	55.0	50.0	57.9	58.8	782-811 bp	1.0	75.8
rpl20-rps12_p4	TCTCCGAGCGATATATCCCC	GCGTCGAGGAACATGTACTA	55.0	50.0	57.9	57.2	779-808 bp	1.0	75.8
rpl20-rps12_p5	TTCTACGTCTCCGAGCGATA	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.8	58.7	788-817 bp	1.0	75.2