Hotspot

trnN-GUU-trnR-ACG

Genome · 145111-145821 · 711 bp

Back to Araucaria

Pi 0.0183

MarkerSeek score 64.60

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: Genome
length_bp: 711
variable_sites: 12
pi: 0.0183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	ACTGACTGGTCGTAGGTTCA	AGAGGATTAGAGCACGTGGT	50.0	50.0	58.0	58.1	774-808 bp	1.0	84.0
trnN-GUU-trnR-ACG_p2	ACTGACTGGTCGTAGGTTCA	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.0	57.8	775-809 bp	1.0	83.4
trnN-GUU-trnR-ACG_p3	GCTGTTAACTGACTGGTCGT	AGAGGATTAGAGCACGTGGT	50.0	50.0	57.8	58.1	781-815 bp	1.0	82.8
trnN-GUU-trnR-ACG_p4	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	782-816 bp	1.0	82.3
trnN-GUU-trnR-ACG_p5	TTCCTCAGTAGCTCAGTGGT	AGAGGATTAGAGCACGTGGT	50.0	50.0	57.7	58.1	811-845 bp	1.0	81.5