Hotspot

trnI-CAU-trnD

Genome · 120125-120835 · 711 bp

Back to Araucaria

Pi 0.0113

MarkerSeek score 65.20

Primer available yes

Label trnI-CAU-trnD

Feature Metadata

label_name: trnI-CAU-trnD
feature_type: igs
region: Genome
length_bp: 711
variable_sites: 8
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-trnD_p1	AATTGAACCCGTGACTTCCC	TTAGAGTACCGCCCTGTCAA	50.0	50.0	58.1	58.1	825 bp	1.0	79.4
trnI-CAU-trnD_p2	GAACCCGTGACTTCCCAATT	TTAGAGTACCGCCCTGTCAA	50.0	50.0	58.1	58.1	821 bp	1.0	79.4
trnI-CAU-trnD_p3	AATTGAACCCGTGACTTCCC	ATTGGTTAGAGTACCGCCCT	50.0	50.0	58.1	58.1	830 bp	1.0	79.0
trnI-CAU-trnD_p4	GAACCCGTGACTTCCCAATT	ATTGGTTAGAGTACCGCCCT	50.0	50.0	58.1	58.1	826 bp	1.0	79.0
trnI-CAU-trnD_p5	CAGGAATTGAACCCGTGACT	TTAGAGTACCGCCCTGTCAA	50.0	50.0	57.8	58.1	829 bp	1.0	78.7