Hotspot

trnW-CCA-petG

Genome · 101622-101739 · 118 bp

Back to Araucaria

Pi 0.0424

MarkerSeek score 70.40

Primer available yes

Label trnW-CCA-petG

Feature Metadata

label_name: trnW-CCA-petG
feature_type: igs
region: Genome
length_bp: 118
variable_sites: 5
pi: 0.0424
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-petG_p1	AGCGCTCTTAGTTCAGTTCG	ATTGAGGCTTTGCTATCCGG	50.0	50.0	58.0	58.0	304 bp	1.0	81.0
trnW-CCA-petG_p2	CCTATCCGGGCTTCTCCTAA	ATTGAGGCTTTGCTATCCGG	55.0	50.0	58.0	58.0	327 bp	1.0	80.9
trnW-CCA-petG_p3	GCTTCTCCTAAGAAAGCGCT	ATTGAGGCTTTGCTATCCGG	50.0	50.0	58.0	58.0	318 bp	1.0	80.9
trnW-CCA-petG_p4	TCGGTAGAACGTAGGTCTCC	ATTGAGGCTTTGCTATCCGG	55.0	50.0	58.0	58.0	287 bp	1.0	80.9
trnW-CCA-petG_p5	TCCGGGCTTCTCCTAAGAAA	ATTGAGGCTTTGCTATCCGG	50.0	50.0	58.1	58.0	323 bp	1.0	80.6