Hotspot

psaM-trnG-UCC

Genome · 38792-39360 · 569 bp

Back to Araucaria

Pi 0.016

MarkerSeek score 68.50

Primer available yes

Label psaM-trnG-UCC

Feature Metadata

label_name: psaM-trnG-UCC
feature_type: igs
region: Genome
length_bp: 569
variable_sites: 9
pi: 0.016
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaM-trnG-UCC_p1	AAAGCAGCAACAAGGGCTAT	TGCGAGTTAATGATGGAACGA	45.0	42.9	58.1	57.8	658-664 bp	1.0	71.5
psaM-trnG-UCC_p2	AAAAGCAGCAACAAGGGCTA	TGCGAGTTAATGATGGAACGA	45.0	42.9	58.3	57.8	659-665 bp	1.0	69.6
psaM-trnG-UCC_p3	TAAAAGCAGCAACAAGGGCT	TGCGAGTTAATGATGGAACGA	45.0	42.9	58.3	57.8	660-666 bp	1.0	69.6
psaM-trnG-UCC_p4	AAGCAGCAACAAGGGCTATC	TGCGAGTTAATGATGGAACGA	50.0	42.9	58.5	57.8	657-663 bp	1.0	67.8
psaM-trnG-UCC_p5	AAAGCAGCAACAAGGGCTAT	TGCGAGTTAATGATGGAACG	45.0	45.0	58.1	56.3	658-664 bp	1.0	67.4