Hotspot

rps7-ndhB

Genome · 12771-13710 · 940 bp

Back to Araucaria

Pi 0.0196

MarkerSeek score 63.00

Primer available yes

Label rps7-ndhB

Feature Metadata

label_name: rps7-ndhB
feature_type: igs
region: Genome
length_bp: 940
variable_sites: 18
pi: 0.0196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-ndhB_p1	GCAGAGGCCAATAGAGCTTT	TCCATTGAAGAGAAGCAAATGA	50.0	36.4	58.0	56.0	988-1009 bp	1.0	50.7
rps7-ndhB_p2	TTAATGGACGCTGCCAGAAA	TCCATTGAAGAGAAGCAAATGA	45.0	36.4	57.8	56.0	1051-1072 bp	1.0	49.4
rps7-ndhB_p3	GCAAGAAGGAGGAAACCCAT	TCCATTGAAGAGAAGCAAATGA	50.0	36.4	57.8	56.0	1014-1035 bp	1.0	49.3
rps7-ndhB_p4	GCTTTTGCGCATTACCGTTA	TCCATTGAAGAGAAGCAAATGA	45.0	36.4	57.7	56.0	973-994 bp	1.0	49.2
rps7-ndhB_p5	TAATGGACGCTGCCAGAAAG	TCCATTGAAGAGAAGCAAATGA	50.0	36.4	58.3	56.0	1050-1071 bp	1.0	48.9