Hotspot

rrn16-trnV-GAC

Genome · 8829-9888 · 1060 bp

Back to Araucaria

Pi 0.028
MarkerSeek score 68.70
Primer available yes
Label rrn16-trnV-GAC

Feature Metadata

label_name
rrn16-trnV-GAC
feature_type
igs
region
Genome
length_bp
1060
variable_sites
29
pi
0.028
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn16-trnV-GAC_p1 TCCGACTTGCATGTGTTAGG TTGACGTGGTGATGGTCATC 50.0 50.0 57.9 57.9 1160-1169 bp 1.0 82.1
rrn16-trnV-GAC_p2 TCCGACTTGCATGTGTTAGG CTTGACGTGGTGATGGTCAT 50.0 50.0 57.9 57.9 1161-1170 bp 1.0 82.0
rrn16-trnV-GAC_p3 TCCGACTTGCATGTGTTAGG GATGGTCATCGGTTCGAGTC 50.0 55.0 57.9 58.2 1150-1159 bp 1.0 81.6
rrn16-trnV-GAC_p4 GCAGGTTCTTACGCGTTACT TTGACGTGGTGATGGTCATC 50.0 50.0 58.3 57.9 1212-1221 bp 1.0 80.3
rrn16-trnV-GAC_p5 GCAGGTTCTTACGCGTTACT CTTGACGTGGTGATGGTCAT 50.0 50.0 58.3 57.9 1213-1222 bp 1.0 80.3